艾叶水提液增强家兔抗大肠杆菌免疫的研究

doi:10.11843/j.issn.0366-6964.2019.11.020

摘要/Abstract

摘要： 用艾叶水提液给家兔饮水，通过ELISA技术和攻毒试验评价其对灭活大肠杆菌诱导特异性抗体反应和免疫保护作用的影响；利用ELISA、血常规、血生化、定量显色基质法以及RT-PCR等技术，对给药动物抗大肠杆菌（E.coli）固有免疫作用进行评估。结果显示，与对照组比较，艾叶饮水7 d能极显著增强灭活大肠杆菌诱导的血清特异性IgM、IgG抗体应答（P<0.01），且明显提高免疫动物感染E.coli后存活率；艾叶单独饮水能显著升高血清总IgG、IgA抗体水平（P<0.05），增加血液中性粒细胞（P<0.05）和血小板的数量（P<0.01），明显减少E.coli攻毒12 h后血液中E.coli数量（P<0.05）、LPS浓度（P<0.01）以及谷草转氨酶和谷丙转氨酶的含量（P<0.05）。另外，口服艾叶水提液能显著降低E.coli攻毒所致与LPS-TLR4通路相关的细胞因子（TLR4、TNF-α、IL-1β、IRF7、IFN-β） mRNA的过度表达（P<0.01或P<0.05）。结果表明，艾叶是一种优良的抗E.coli免疫增强剂，具有临床应用的潜力。

Abstract: Rabbits were oral administrated with water extract of Artemisia argyi Levl. et Vant. (Artemisia argyi),subsequently the animals were injected with or without inactivated E. coli. After that,an indirect ELISA was used to detect the specific antibody induced by inactivated E. coli, and challenge test was conducted to assess the immune protection effect induced by inactivated E.coil. In addition,the innate immunity of rabbits against E. coli were measured by the methods of ELISA,blood routine,blood biochemistry,limulus amoebocyte lysate test with a chromogenic substrate,and RT-PCR. The results showed that the improved level of specific IgM, IgG (P<0.01), and the survival of rabbits challenged with E. coli were found in the animals administrated with Artemisia argyi for 7 days as compared to the control. More importantly, the supplement of Artemisia argyi alone clearly increased the total IgG, IgA (P<0.05),and the number of neutrophils(P<0.05), platelets(P<0.01) in blood, while along with the reduced viable cells of E. coli (P<0.05), LPS level (P<0.01),AST and ALT (P<0.05) were observed in blood of animals challenged with E. coli. Additionally,it was observed in the drug group that the mRNA expression of cytokines (TLR4, TNF-α, IL-1β, IRF7, IFN-β) which were correlated with the LPS-TLR4 signaling pathway and over-expressed after challenge with E. coli were significantly down-regulated (P<0.01 or P<0.05). Collectively,Artemisia argyi is an excellent immunopotentiator to increase rabbits resistance to E. coli, and has its potential clinical application value.

中图分类号:

S853.74

王华, 张鲁滨, 张雪冰, 任美玉, 杨小燕, 倪敬轩, 周孝琼. 艾叶水提液增强家兔抗大肠杆菌免疫的研究[J]. 畜牧兽医学报, 2019, 50(11): 2348-2356.

WANG Hua, ZHANG Lubin, ZHANG Xuebing, REN Meiyu, YANG Xiaoyan, NI Jingxuan, ZHOU Xiaoqiong. Enhancement of Rabbit Immunity against E. coli by Oral Administration of Water Extract of Artemisia argyi Levl. et Vant.[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2348-2356.

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